Plasmid_Backbone

Part:BBa_M36099:Design

Designed by: Joseph Shih   Group: Stanford BIOE44 - S11   (2013-04-23)

E. coli sensor test rig


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2342
    Illegal XbaI site found at 3188
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2342
    Illegal XbaI site found at 3188
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2342
    Illegal XbaI site found at 3188
    Illegal AgeI site found at 2605
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

Cloning Cassettes 1 and 2 used for inserting parts into an empty vector. Parts in between the cloning cassettes were designed by BIOE44. The parts including and outside of the cloning cassettes were provided by DNA2.0.

rpo_bla and rrnB1T1T2 transcription terminators serve as insulators.

Source

Comet GFP from DNA2.0 The rest of the vector is sourced from DNA2.0.

References